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LeCDJ1, a chloroplast DnaJ protein, facilitates heat tolerance in transgenic tomatoes
Author(s) -
Kong Fanying,
Deng Yongsheng,
Wang Guodong,
Wang Jieru,
Liang Xiaoqing,
Meng Qingwei
Publication year - 2014
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12119
Subject(s) - apx , superoxide dismutase , peroxidase , photosystem ii , photoinhibition , chemistry , malondialdehyde , abscisic acid , biochemistry , catalase , chloroplast , hydrogen peroxide , antioxidant , photosynthesis , enzyme , gene
The roles of a tomato ( L ycopersicon e sculentum ) c hloroplast‐targeted D na J protein (LeCDJ1) were investigated using wild‐type (WT) and sense transgenic tomatoes. The LeCDJ1 expression was upregulated by 38 °C, 42 °C, 45 °C, NaCl, PEG, methyl viologen (MV) and hydrogen peroxide (H 2 O 2 ), but not by 30 °C and 35 °C. Meanwhile, LeCDJ1 was involved in the response of plants to abscisic acid (ABA). Under heat stress, the sense plants showed better growth, higher chlorophyll content, lower malondialdehyde (MDA) accumulation and relative electrical conductivity (REC), and also less PSII photoinhibition than WT. Interestingly, the sense plants treated with streptomycin (SM), an inhibitor of organellar translation, still showed higher maximum photochemistry efficiency of PSII (Fv/Fm) and D1 protein levels than the SM‐untreated WT, suggesting that the protective effect of LeCDJ1 on PSII was, at least partially, independent of D1 protein synthesis. Furthermore, the relatively lower superoxide radical (O 2 •− ) and H 2 O 2 levels in the sense plants were considered to be due to the higher ascorbate peroxidase (APX) and superoxide dismutase (SOD) activity, which seemed unlikely dependent on their transcription level. These results indicated that LeCDJ1 overexpression facilitated heat tolerance in transgenic tomatoes.