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Characterization and Mapping of a Salt‐Sensitive Mutant in Rice ( Oryza sativa L.)
Author(s) -
Zhou Jingwei,
Wang Fuzheng,
Deng Ping,
Jing Wen,
Zhang Wenhua
Publication year - 2013
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12048
Subject(s) - ethyl methanesulfonate , quantitative trait locus , biology , oryza sativa , locus (genetics) , genetics , mutant , population , gene mapping , positional cloning , indel , chromosome 4 , gene , chromosome , genetic analysis , chromosome 3 , genotype , single nucleotide polymorphism , demography , sociology
A salt‐sensitive mutant designated rice salt sensitive 2 ( rss2 ) was isolated from the M 2 generation of the rice cultivar Nipponbare mutagenized with ethyl methanesulfonate (EMS). This mutant exhibited a greater decrease in salt tolerance with a significant increase in Na + content in its shoots. Genetic analysis indicated that the increase in Na + in rss2 was controlled by a single recessive gene. Further genome‐wide analysis of the linkage map constructed from the F 2 population of rss2 /Zhaiyeqing 8 (ZYQ8) showed that two quantitative trait loci (QTLs) on chromosomes 1 and 6 were responsible for the Na + concentration in shoots, which explained 14.5% and 53.3%, respectively, of the phenotypic variance. The locus on chromosome 1, but not that on chromosome 6, was also detected in the F 2 population of Nipponbare/ZYQ8, suggesting that the QTL on chromosome 6 was responsible for the salt sensitivity in rss2 . By analyzing the recombination events in 220 mutant individuals of an enlarged mapping population of rss2 /ZYQ8, the rss2 locus was precisely mapped to an interval of 605.3 kb between insertion/deletion (InDel) markers IM21962 and IM22567. This finding will facilitate the cloning of the rss2 locus and provide insight into the physiological mechanisms of salt sensitivity in rice.