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Small Ubiquitin‐Like Modifier Conjugating Enzyme with Active Site Mutation Acts as Dominant Negative Inhibitor of SUMO Conjugation in Arabidopsis F
Author(s) -
Tomanov Konstantin,
Hardtke Christian,
Budhiraja Ruchika,
Hermkes Rebecca,
Coupland George,
Bachmair Andreas
Publication year - 2013
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12016
Subject(s) - arabidopsis , ubiquitin , enzyme , mutation , biochemistry , chemistry , ubiquitin conjugating enzyme , biology , genetics , ubiquitin ligase , mutant , gene
Small ubiquitin‐like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss‐of‐function mutants.