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Gut microbiome diversity in acute severe colitis is distinct from mild to moderate ulcerative colitis
Author(s) -
Kedia Saurabh,
Ghosh Tarini Shankar,
Jain Saransh,
Desigamani Anbumani,
Kumar Ashok,
Gupta Vipin,
Bopanna Sawan,
Yadav Dawesh P,
Goyal Sandeep,
Makharia Govind,
Travis Simon P. L.,
Das Bhabatosh,
Ahuja Vineet
Publication year - 2021
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.15232
Subject(s) - microbiome , ulcerative colitis , colitis , medicine , cohort , gastroenterology , cohort study , gut microbiome , gut flora , immunology , biology , bioinformatics , disease
Abstract Background and Aim Although the gut microbiome of patients with ulcerative colitis (UC) has been characterized, no study has characterized the gut microbiome in acute severe colitis (ASC). We compared the gut microbiome of patients with UC, ASC, and healthy controls (HCs). Methods Patients with mild to moderate UC ( n = 24), ASC ( n = 19 with 21 episodes) and HCs ( n = 50) were recruited prospectively. A 16SrDNA amplicon approach was used to explore gut microbial diversity and taxonomic repertoires. UC was diagnosed using European Crohn's and Colitis Organization guidelines, and ASC was diagnosed using Truelove and Witts' criteria. Results The normalized alpha diversity was significantly lower in ASC than mild–moderately active UC ( P < 0.05) or HC ( P < 0.001). The gut microbiome in ASC was highly unstable, as characterized by high intracohort variation (analyzed using J‐divergence measure), which was significantly greater than in UC or HC. On principal coordinate analysis, the microbiome of HC and UC were similar, with the ASC cohort being distinct from both. Comparison of ranked abundances identified four distinct clusters of genera (G1, G2, G3, and G4), with specific trends in their abundance across three groups: G1/G2A clusters had the least, whereas G3 had the highest abundance in the ASC cohort. Conclusions Gut microbial diversity is lower in ASC than mild–moderate UC or HCs. Gut microbiome composition is increasingly unstable in ASC, with a distinct abundance of specific genera varying between HCs and ASC. Mild–moderate UC lies within the spectrum.