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Modulations of probiotics on gut microbiota in a 5‐fluorouracil‐induced mouse model of mucositis
Author(s) -
Yeung ChunYan,
Chiang Chiau JenShiu,
Cheng MeiLein,
Chan WaiTao,
Chang SzuWen,
Chang YuanHao,
Jiang ChuenBin,
Lee HungChang
Publication year - 2020
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.14890
Subject(s) - gut flora , lactobacillus reuteri , mucositis , microbiology and biotechnology , probiotic , biology , lactobacillus , lactobacillus casei , bacteroides , bacteroides fragilis , lactobacillus rhamnosus , immunology , medicine , antibiotics , chemotherapy , bacteria , genetics
Background and Aim Intestinal mucositis remained one of the most deleterious complications in cancer patients undergoing chemotherapy. 5‐FU treatment was reported to affect the abundance of gut microbiota and cause mucositis, which might be ameliorated by probiotics. We investigate the potential changes of 5‐FU treatment and the modulations of probiotics on gut microbiota in a mouse model. Methods Male BALB/c mice received either 5‐FU or saline (S). They were separated and fed saline, Lactobacillus casei variety rhamnosus (Lcr) and Lactobacillus reuteri DSM 17938 (BG). Lcr and BG were simultaneously administered with 5‐FU for 5 days. Stool specimens were collected for DNA extraction and pyrosequenced for bioinformatic analysis. Results Fecal microbial communities were obviously diverse. Bacteroides and Bacteroidaceae were the most abundant microbiota in FU.BG group while S24_7 was the most in S.S group. At phylum and class levels, abundances of Betaproteobacteria, Erysipelotrichi, Gammaproteobacteria, and Verrucomicrobia were significantly increased in the FU groups. Probiotics supplementation did increase the abundances of Enterobacteriales and Turicibacterales. We demonstrated that probiotics did modulate the abundance and diversity of gut microbiota. Bacterial motility proteins were found enriched and upregulated in the S.BG group. No mortality was noted. No bacterial translocation was found in spleen and blood among the six groups. Conclusion Gut microbiota of mice undergoing chemotherapy exhibited a distinct disruption in bacterial composition. Probiotic did modulate the abundance and diversity of gut microbiota. This is the first study to analyze the effects and safety of Lactobacillus strains on 5‐FU‐induced mucositis systematically and assess changes in the intestinal microbiota after probiotic intervention.