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Caudal type homeobox 2 expression induced by leukocytapheresis might be associated with mucosal healing in ulcerative colitis
Author(s) -
Tsuchiya Kiichiro,
Hayashi Ryohei,
Fukushima Keita,
Hibiya Shuji,
Horita Nobukatsu,
Negi Mariko,
Itoh Eisaku,
Akashi Takumi,
Eishi Yoshinobu,
Motoya Satoshi,
Takeuchi Yoshiaki,
Kunisaki Reiko,
Fukunaga Ken,
Nakamura Shiro,
Yoshimura Naoki,
Takazoe Masakazu,
Iizuka Bunei,
Suzuki Yasuo,
Nagahori Masakazu,
Watanabe Mamoru
Publication year - 2017
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.13645
Subject(s) - cdx2 , medicine , ulcerative colitis , homeobox , biopsy , goblet cell , pathology , immunology , gastroenterology , cancer research , transcription factor , epithelium , disease , biology , gene , biochemistry
Background and Aim Ulcerative colitis (UC) is a chronic inflammatory disease of the colon with an intractable, recurrent course. Although the goal of UC therapy has recently been to target mucosal healing, the molecular mechanism of mucosal healing remains unknown. In this study, we aimed to elucidate the molecular dynamics related to the proliferation and differentiation of intestinal epithelial cells during cytapheresis therapy in a short duration. Methods Endoscopy was performed in 26 patients with UC in multicentre hospitals, and biopsy specimens were collected from the rectum before and within two weeks after leukocytapheresis (LCAP). The expression of representative proteins in intestinal epithelial cells and pathological findings was compared before and after LCAP. Results The expression of caudal type homeobox 2 (CDX2) and a hes family bHLH transcription factor 1(HES1) markedly increased after LCAP. Patients with endoscopic improvement after LCAP showed the expression of CDX2 before LCAP. Moreover, the number of goblet cells significantly increased after LCAP. Patients without endoscopic improvement after LCAP did not show the expression of CDX2 before LCAP. However, the expression of CDX2 markedly increased after LCAP. Conclusion This study suggests that cytapheresis might induce CDX2 expression without affecting the cell proliferation, thus resulting in mucosal healing with goblet cell restoration.