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Proprotein convertase 7 rs236918 associated with liver fibrosis in Italian patients with HFE ‐related hemochromatosis
Author(s) -
Pelucchi Sara,
Galimberti Stefania,
Greni Federico,
Rametta Raffaela,
Mariani Raffaella,
Pelloni Irene,
Girelli Domenico,
Busti Fabiana,
Ravasi Giulia,
Valsecchi Maria Grazia,
Valenti Luca,
Piperno Alberto
Publication year - 2016
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.13315
Subject(s) - medicine , gastroenterology , cirrhosis , odds ratio , genotype , hemochromatosis , fibrosis , hepatocellular carcinoma , liver biopsy , penetrance , hereditary hemochromatosis , single nucleotide polymorphism , pathology , biopsy , biology , genetics , phenotype , gene
Background and Aim p.Cys282Tyr homozygosity is the prevalent genotype in ( HFE )‐related Hereditary Hemochromatosis with low penetrance and variable expression. However, liver cirrhosis and hepatocellular carcinoma remain the main causes of mortality in these patients. Detection of genetic modifiers identifying patients at risk for liver damage would be relevant for their clinical management. We evaluated proprotein convertase 7 ( PCSK7 ) rs236918 as genetic marker of risk of liver fibrosis in an Italian cohort of p.Cys282Tyr homozygotes. Methods Liver fibrosis was histologically assessed by Ishak score. We evaluated PCSK7 alleles and genotypes frequencies according to single or grouped staging scores: absent/mild fibrosis (stage: 0–2), moderate (stage: 3–4), and severe fibrosis/cirrhosis (stage: 5–6). Single nucleotide polymorphism genotyping was performed by restriction fragment length polymorphism or Taqman 5′‐nuclease assays. Results The rs236918 allele C frequency increased from stages 0–2 to 5–6 (7.1% vs 13.6%, vs 21.9%, P = 0.003). The wild‐type genotype was significantly more frequent in the absent/mild fibrosis group (54.2%) compared with only 17% in patients with severe fibrosis/cirrhosis. At univariate proportional odds model, patients with GC + CC genotypes were 2.77 times ( P = 0.0018) more likely to have worse liver staging scores than wild‐type patients. In the adjusted analysis, odds ratio was 2.37 ( P = 0.0218), and 2.56 ( P = 0.0233) when the analysis was restricted to males. An exploratory mediation analysis suggested a direct effect of genotype on severe fibrosis/cirrhosis (odds ratio = 3.11, P = 0.0157), and a mild non‐significant indirect effect mediated through iron accounting for 28%. Conclusions These findings confirm that PCSK7 rs236918 C allele is a risk factor for cirrhosis development in Italian patients with HFE ‐Hemochromatosis.