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Association of vitamin D receptor gene polymorphisms and serum 25‐hydroxyvitamin D levels with Crohn's disease in Chinese patients
Author(s) -
Xia Shenglong,
Lin Xinxin,
Guo Maodong,
Zhang Daguan,
Zheng Shuzi,
Jiang Lijia,
Jin Jie,
Lin Xiuqing,
Ding Ran,
Jiang Yi
Publication year - 2016
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.13221
Subject(s) - taqi , foki , calcitriol receptor , medicine , vitamin d and neurology , endocrinology , genotype , haplotype , crohn's disease , vitamin d deficiency , gastroenterology , immunology , polymorphism (computer science) , biology , disease , gene , genetics
Background and Aim: The vitamin D receptor (VDR) regulates immune responses and inflammation through binding with 1,25‐dihydroxyvitamin D, the active form of vitamin D. The serum 25‐hydroxyvitamin D (25(OH)D) level clinically reflects vitamin D status in the human body. We investigated the association of VDR polymorphisms and 25(OH)D levels in Chinese patients with Crohn's disease (CD). Methods: Vitamin D receptor polymorphisms ( Fok I, Bsm I, Apa I, and Taq I) were genotyped by SNaPshot . Serum 25(OH)D levels were measured by electro‐chemiluminescence immunoassay. Results: A total of 297 patients with CD and 446 controls were recruited. Compared with controls, mutant alleles and genotypes of Bsm I and Taq I were less prevalent in patients with CD (all P < 0.05/4 = 0.0125). The AAC haplotype formed by Bsm I, Apa I, and Taq I was also less prevalent in patients with CD ( P = 0.004). Furthermore, 124 patients and 188 controls were randomly selected for measurements of 25(OH)D levels. Average 25(OH)D level was lower in patients with CD than in controls (15.46 ± 8.11 vs 21.64 ± 9.45 ng/mL, P < 0.001) and negatively linked to CD activity index ( β = −0.829, P < 0.001), platelet count ( β = −0.253, P < 0.001) and neutrophil percentage ( β = −0.136, P = 0.005) in patients with CD. The Apa I mutant genotype and vitamin D deficiency (<20 ng/mL) were independently associated with CD ( P = 0.009, P < 0.001, respectively). In patients with CD, vitamin D deficiency interacted with Fok I, Apa I, and Taq I mutant genotypes ( P = 0.027, P = 0.024, and P = 0.040, respectively). Conclusions: Vitamin D receptor ( Bsm I, Apa I, and Taq I) mutations and lower 25(OH)D levels are associated with CD in Chinese patients. Moreover, VDR ( Fok I, Apa I, and Taq I) mutations and vitamin D deficiency may have a combined impact on CD.