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Pretransplant replacement of donor liver grafts with recipient K upffer cells attenuates liver graft rejection in rats
Author(s) -
Endo Kosuke,
Hori Tomohide,
Jobara Kanta,
Hata Toshiyuki,
Tsuruyama Tatsuaki,
Uemoto Shinji
Publication year - 2015
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12872
Subject(s) - medicine , liver transplantation , staining , transplantation , urology , pathology , gastroenterology
Abstract Background and Aim Rejection of liver grafts is a difficult issue that has not been resolved. Preoperative replacement of liver cells in the graft with cells from the intended recipient may attenuate rejection. We investigated whether preoperative transplant of recipient bone marrow cells ( BMC s) to the donor replaced liver allograft cells and attenuated rejection. Methods We used a rat model of allogeneic liver transplant ( LT ) from D ark A gouti ( DA ) to L ewis ( LEW ) rats. In BMC group, DA rats received BMC transplants from LacZ ‐transgenic LEW rats at 1 week before LT . In the control group, DA rats received no preoperative treatment. We evaluated graft damage at 7 days after LT and the survival of the recipient rats. Results Rats in the BMC group experienced prolonged survival that was abrogated by the administration of gadolinium chloride to donors at 24 h before LT . Serum concentrations of total bilirubin and hyaluronic acid on day 7 were significantly lower in the BMC group, and histopathological analyses revealed that rejection of the liver graft was attenuated. X ‐gal staining and immunohistostaining of the liver graft revealed that BMC s engrafted in the sinusoidal space differentiated into K upffer cells. Conclusions Preoperative transplant of recipient BMC s to LT donors replaced donor K upffer cells and attenuated post‐ LT rejection, indicating that this strategy may increase the success of LT .