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Association between promoter methylation and expression of thyroid hormone receptor beta ( THR β ) gene in patients with gastric cancer in an I ranian population
Author(s) -
Kordi Tamandani Dor Mohammad,
Hemati Simin,
Davani Shahrbbanou Karimi,
Arbabi Farshid
Publication year - 2015
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12808
Subject(s) - methylation , medicine , cancer , promoter , thyroid hormone receptor , thyroid , thyroid cancer , population , cancer research , polymerase chain reaction , hormone receptor , real time polymerase chain reaction , gene expression , gene , microbiology and biotechnology , oncology , biology , breast cancer , genetics , environmental health
Background and Aim There is evidence that gastric cancer patients suffer from thyroid disorders. However, the relationship between thyroid receptor ( TR ) expression and gastric cancer remains unknown. The aim of this study was to evaluate the status of promoter methylation and expression of the thyroid hormone receptor beta ( THR β ) gene in gastric cancer patients in an Iranian population. Methods Analysis of THR β promoter methylation was performed on 85 pairs of formalin‐fixed, paraffin‐embedded ( FFPE ) tissue samples as cases and controls via methylation‐specific polymerase chain reaction ( PCR [ MSP ]). The samples were obtained from tumors and surrounding healthy tissues from resected gastric cancers. The expression assay was also performed with 25 FFPE tissue pairs (tumor and surrounding healthy tissues of the same individual) using real‐time PCR . Results The results of the present study show that there is a statistically significant difference between tumor and adjacent normal tissues regarding promoter methylation status and THR β expression ( P = 0.04 and P = 0.036, respectively). Conclusion Therefore, promoter methylation of THR β may be involved in the development of gastric cancer.