Premium
Differential stimulation of peripheral blood mononuclear cells in C rohn's disease by fungal glycans
Author(s) -
Baram Liran,
CohenKedar Sarit,
Spektor Lior,
Elad Hofit,
GuznerGur Hanan,
Dotan Iris
Publication year - 2014
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12701
Subject(s) - peripheral blood mononuclear cell , mannan , immune system , immunology , cytokine , medicine , glycan , tumor necrosis factor alpha , biology , microbiology and biotechnology , glycoprotein , polysaccharide , biochemistry , in vitro
Abstract Background and Aim C rohn's disease ( CD ) is characterized by loss of tolerance to intestinal microorganisms. This is reflected by serological responses to fungal glycans such as mannan and β‐glucans. Fungal glycans have various effects on immune cells. However, the evidence for their effects in CD is vague. This study aimed to assess the effects of fungal cell wall glycans on human peripheral blood mononuclear cells ( PBMC s) from CD and control patients. Methods Human PBMC s from CD and control patients were stimulated by fungal cell wall glycans. Cytokine secretion was detected by ELISA and glycan receptor expression by flow cytometry. Results Mannan, β‐glucans (curdlan), chitosan, and zymosan induced the secretion of interleukin ( IL )‐1β, IL ‐6, IL ‐23, IL ‐10, and tumor necrosis factor‐α by PBMC s. Spleen tyrosin kinase and S rc tyrosine kinase were involved in the response to mannan and β‐glucans. Mannan and whole yeast cells induced a significantly higher pro‐inflammatory cytokine response in CD compared with control patients. Conclusions The results may suggest that CD is characterized by hyperresponsiveness to fungal glycans. Thus, glycans may potentially be triggering or perpetuating inflammation.