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Association between specific mucosa‐associated microbiota in Crohn's disease at the time of resection and subsequent disease recurrence: A pilot study
Author(s) -
De Cruz Peter,
Kang Seungha,
Wagner Josef,
Buckley Michael,
Sim Winnie H,
Prideaux Lani,
Lockett Trevor,
McSweeney Chris,
Morrison Mark,
Kirkwood Carl D,
Kamm Michael A
Publication year - 2015
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12694
Subject(s) - crohn's disease , firmicutes , medicine , prevotella , gastroenterology , colonoscopy , gut flora , veillonella , disease , bacteroides , ileum , microbiome , cecum , streptococcus , immunology , colorectal cancer , biology , bioinformatics , cancer , bacteria , 16s ribosomal rna , genetics
Background and Aim C rohn's disease pathogenesis involves alterations in the gut microbiota. We characterized the mucosa‐associated microbiota at the time of surgical resection and 6 months later to identify bacterial profiles associated with recurrence and remission. Methods Tissue samples were collected from surgical resection specimens in 12 C rohn's disease patients, and at 6 months postoperative colonoscopy from the neoterminal ileum and anastomosis. Endoscopic recurrence was assessed using the R utgeerts score. Microbiota was characterized using microarray and 454 pyrosequencing. Longitudinal comparisons were made within patients, and cross‐sectional comparisons made with colonoscopic biopsies from the terminal ileum and cecum of 10 healthy subjects. Results Microbiota of healthy subjects had high diversity and was dominated by the F irmicutes, B acteroidetes, and P roteobacteria phyla. Biodiversity was lower in C rohn's disease patients at the time of surgery, increased after surgery, but still differed from healthy subjects. C rohn's disease patients with recurrent disease retained a microbiota favoring proteolytic‐fueled fermentation and lactic acid‐producing bacteria, including E nterococcus and V eillonella spp., while those maintaining remission demonstrated predominant saccharolytic B acteroides , P revotella , and P arabacteroides spp., and saccharolytic, butyrate‐producing F irmicutes. Conclusion In C rohn's disease, the mucosa‐associated microbiota diversity is reduced at the time of surgery, but also differs between patients with different clinical outcomes at 6 months. These findings may provide prognostic information at the time of surgery, allowing identification of patients at increased risk of recurrence, and provide basis for a more targeted approach for therapeutic interventions after surgery.