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Clinical utility of a simple primary culture method in hepatocellular carcinoma patients
Author(s) -
Lin ZuYau,
Wu ChunChieh,
Chuang YenHwang,
Chuang WanLong
Publication year - 2015
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12693
Subject(s) - medicine , hepatocellular carcinoma , liver cancer , cancer , incidence (geometry) , carcinoma , cancer cell , oncology , gastroenterology , pathology , physics , optics
Background and Aim The clinical utility of our designed primary culture method in patients with hepatocellular carcinoma was investigated. Methods Specimens obtained from ultrasound‐guided fine‐needle aspiration of 108 hepatocellular carcinoma patients were cultured. The associations of the cellular proliferative speeds with cancer invasiveness and 1‐year survivals were analyzed. Results Successful cultures were achieved in 105 patients (97.2%). Ten hepatocellular carcinoma and nine cancer‐associated fibroblast cell lines were established. The cells obtained from patients with A merican J oint C ommittee on C ancer TNM staging ≥ IIIB upon entering the study had higher proportion of rapidly proliferative cancer cells than those from patients with staging ≤ IIIA ( P < 0.005). For B arcelona C linic L iver C ancer classification A or B patients receiving palliative transcatheter chemoembolization, patients with rapidly proliferative cancer‐associated fibroblasts showed higher incidence of cancer‐related death than patients with other proliferative patterns ( P = 0.0385). The influence of the presence of rapidly proliferative cancer cells on survivals in this group could not be calculated due to a very small number of this kind of patients (9.5%). For B arcelona C linic L iver C ancer classification C patients receiving non‐curative treatment, the incidence of rapidly proliferative cancer cells was 45.2%. Patients with rapidly proliferative cancer cells showed higher incidence of cancer‐related death than patients with other proliferative patterns in patients receiving chemoembolization ( P = 0.0452) and in patients receiving conservative treatment ( P = 0.0206). Conclusion Our method can provide cells from individual patient for researches and predict outcomes in patients receiving non‐curative treatment of hepatocellular carcinoma.