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Gene profile in the spleen under massive partial hepatectomy using complementary DNA microarray and pathway analysis
Author(s) -
Arakawa Yusuke,
Shimada Mitsuo,
Utsunomiya Tohru,
Imura Satoru,
Morine Yuji,
Ikemoto Tetsuya,
Mori Hiroki,
Kanamoto Mami,
Iwahashi Shuichi,
Saito Yu,
Takasu Chie
Publication year - 2014
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12573
Subject(s) - spleen , downregulation and upregulation , microbiology and biotechnology , medicine , gene expression , microarray analysis techniques , microarray , messenger rna , gene , immunology , andrology , biology , genetics
Abstract Background and Aim In general, the spleen is one of the abdominal organs connected by the portal system, and a splenectomy improves hepatic functions in the settings of partial hepatectomy ( Hx ) for portal hypertensive cases or living donor liver transplantation with excessive portal vein flow. Those precise mechanisms remain still unclear; therefore, we investigated the DNA expression profile in the spleen after 90% Hx in rats using complementary DNA microarray and pathway analysis. Methods Messenger RNAs ( mRNAs ) were prepared from three rat spleens at each time point (0, 3, and 6 h after 90% Hx ). Using the gene chip, mRNA was hybridized to A ffymetrix G ene C hip R at G enome 230 2.0 A rray ( A ffymetrix®) and pathway analysis was done with I ngenuity P athway A nalysis ( IPA ®). Results We determined the 3‐h or 6‐h/0‐h ratio to assess the influence of Hx, and cut‐off values were set at more than 2.0‐fold or less than 1/2 (0.5)‐fold. Chemokine activity‐related genes including Cxc l1 ( GRO 1) and Cxc l2 ( MIP ‐2) related pathway were upregulated in the spleen. Also, immediate early response genes including early growth response‐1 ( EGR 1), FBJ murine osteosarcoma ( FOS ) and activating transcription factor 3 ( ATF 3) related pathway were upregulated in the spleen. Conclusions We concluded that in the spleen the expression of numerous inflammatory‐related genes would occur after 90% Hx . The spleen could take a harmful role and provide a negative impact during post Hx phase due to the induction of chemokine and transcription factors including GRO 1 and EGR 1.