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Association of polymorphisms in virulence factor of H elicobacter pylori and gastroduodenal diseases in S outh K orea
Author(s) -
Kim Ji Yeon,
Kim Nayoung,
Nam Ryoung Hee,
Suh Ji Hyung,
Chang Hyun,
Lee Jung Won,
Kim Young Sun,
Kim Jung Mogg,
Choi Jae Won,
Park Jung Geun,
Lee Yeon Suk,
Lee Dong Ho,
Jung Hyun Chae
Publication year - 2014
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12509
Subject(s) - caga , virulence , helicobacter pylori , odds ratio , medicine , genotype , virulence factor , confidence interval , microbiology and biotechnology , gastroenterology , biology , gene , genetics
Background and Aim Clinical outcomes of H elicobacter pylori ( HP ) infection have been shown to be dependent on the variability of virulence factors. The aim of this study was to evaluate the prevalence of each virulence factor and the association between polymorphisms of the virulence factors of HP , and the clinical outcome of gastroduodenal diseases in S outh K orea. Methods Four hundred one HP colonies were analyzed (75 colonies from 45 controls; 71 colonies from 39 benign gastric ulcer [ BGU ] patients; 102 colonies from 54 duodenal ulcer [ DU ] patients; 121 colonies from 77 stomach cancer patients; and 32 colonies from 25 dysplasia patients). Polymerase chain reaction amplifications for vacA , cagA , iceA , oipA , and dupA were performed using DNA extract from HP isolates cultured from mucosal biopsy specimens. dupA was regarded as positive when all of jph0718, jph0719, and dup A were positive. Results Most colonies were composed of vacA s1 (100.0%), i1 (100.0%) and m1 (92.9%), cagA ‐positive (87.2%), iceA 1 (95.8%), oipA ‐positive (91.2%), and dupA ‐negative (52.0%) genotypes. dupA was more frequently expressed in BGU (81.3%), DU (74.7%), and dysplasia (41.7%) than control (16.7%) ( P < 0.001). Infection by dupA ‐positive HP showed an increased risk of BGU (odds ratio 33.06, 95% confidence interval 11.91–91.79) and DU (odds ratio 15.60, 95% confidence interval 6.49–37.49). Conclusion HP infection in S outh K oreans appears to be closely related to highly virulent strains ( vacA s1/i1/m1, cagA (+), iceA 1(+), and oipA (+)), except dupA . dupA has an intimate association with the development of peptic ulcer diseases.