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Phototherapy with artificial light suppresses dextran sulfate sodium‐induced colitis in a mouse model
Author(s) -
Hiratsuka Takahiro,
Inomata Masafumi,
Goto Shigeru,
Oyama Yoshimasa,
Nakano Toshiaki,
Chen ChaoLong,
Shiraishi Norio,
Noguchi Takayuki,
Kitano Seigo
Publication year - 2014
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12460
Subject(s) - medicine , colitis , inflammatory bowel disease , gastroenterology , tumor necrosis factor alpha , ulcerative colitis , immunology , disease
Background and Aim Medical treatment for inflammatory bowel disease ( IBD ) requires chronic administration and causes side effects. Recently, anti‐inflammatory effects of phototherapy were reported in animal models. The present study evaluated whether phototherapy improves dextran sulfate sodium ( DSS )‐induced colitis in a mouse model of IBD . Methods Mice were divided into four equal groups: Control, DSS , DSS + light low ( LL ), and DSS + light high ( LH ) groups. Normal fluorescent light intensity in the Control and DSS groups was 200 lux. Artificial light intensities were as follows: DSS + LL group, 1000 lux; DSS + LH group, 2500 lux. After administering phototherapy for 7 days, we evaluated disease activity index ( DAI ), histological score, colon length/weight, serum 1,25‐dihydroxyvitamin D (3) level, and serum and colonic cytokines in the mice. Results DAI and histological scores were significantly lower in the DSS + LL group than in the DSS group (both, P < 0.05). Colon length and weight were significantly higher in the DSS + LL group than in the DSS group (both, P < 0.05). Serum interleukin ( IL )‐6, TNF ‐α, and IL ‐17 in the DSS + LL group were significantly lower, and serum and colonic IL ‐10 were significantly higher in the DSS + LL group than in the DSS group (all, P < 0.05). Serum 1,25‐dihydroxyvitamin D (3) levels in the DSS + LH group were significantly increased compared with those in the DSS + LL and DSS groups. Conclusion Artificial light phototherapy suppressed DSS ‐induced colitis in mice by suppression of pro‐inflammatory cytokines and promotion of anti‐inflammatory cytokines.