Premium
HLA ‐ DP and γ‐interferon receptor‐2 gene variants and their association with viral hepatitis activity in chronic hepatitis B infection
Author(s) -
Lam YukFai,
Wong Danny KaHo,
Seto WaiKay,
To Kelvin KaiWang,
Hung Ivan FanNgai,
Fung James,
Lai ChingLung,
Yuen ManFung
Publication year - 2014
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12378
Subject(s) - hepatitis b virus , hbeag , viral load , single nucleotide polymorphism , immunology , human leukocyte antigen , genotype , virology , allele , medicine , hepatitis b , interferon , haplotype , odds ratio , virus , biology , gene , antigen , hbsag , genetics
Background and Aim Studies show that polymorphisms in human leukocyte antigen ( HLA )‐ DP loci and certain γ‐interferon ( IFN ‐γ) signaling pathway genes are related to persistence of hepatitis B virus ( HBV ) infection and viral load in chronic HBV ( CHB ) infection respectively. Our study aims to determine whether single‐nucleotide polymorphisms (SNPs) linked to HLA‐DP loci and IFN‐γ signaling pathway are associated with HBV activities.Methods We compared the SNP s in the HLA ‐ DPA 1 gene (rs3077) and the IFN ‐γ receptor‐2 gene (rs2284553 and rs9808753) of 100 treatment‐naive hepatitis B e antigen ( HBeAg )‐negative CHB patients with undetectable HBV DNA with 100 age‐ and sex‐matched controls with HBV DNA > 2000 IU /m L . Results The median age of the study group was 47.9 years, and 61% were male patients. The distribution of the three polymorphisms was in H ardy– W einberg equilibrium. Both rs3077 and rs2284553 polymorphisms were not associated with HBV viral load in terms of allelic frequency, genotypic frequency, dominant/recessive gene action. rs9808753 ( G allele) was associated with a reduced chance of “undetectable HBV DNA ” for patients below the age of 50 years in allelic frequency analysis (odds ratio 0.562; 95% confidence interval, 0.326–0.967; P value = 0.037). IFN ‐γ receptor‐2 gene haplotype block (rs2284553/rs9808753) was not associated with HBV viral activity. Conclusion There was no significant association between HLA ‐ DP polymorphism (rs3077) and IFN ‐γ receptor‐2 gene polymorphism (rs2284553) with viral activity in HBeAg ‐negative CHB patients. Further studies are required to confirm the association between IFN ‐γ receptor‐2 gene polymorphism (rs9808753) and reduced chance of having “undetectable HBV DNA ” in young CHB patients.