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Micro RNA profile before and after antiviral therapy in liver transplant recipients for hepatitis C virus cirrhosis
Author(s) -
Gelley Fanni,
Zadori Gergely,
Nemes Balazs,
Fassan Matteo,
Lendvai Gabor,
Sarvary Eniko,
Doros Attila,
Gerlei Zsuzsanna,
Nagy Peter,
Schaff Zsuzsa,
Kiss Andras
Publication year - 2014
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12362
Subject(s) - medicine , hepatitis c virus , cirrhosis , liver transplantation , hepatitis c , scavenger receptor , receptor , antiviral therapy , gastroenterology , hepacivirus , virus , immunology , transplantation , chronic hepatitis , cholesterol , lipoprotein
Abstract Background and Aim Management of hepatitis C virus ( HCV ) recurrence is a major challenge after liver transplantation. Significant dysregulated expression of HCV receptors (i.e. claudin‐1, occludin, tetraspanin CD 81, scavenger receptor type B1) has been shown recently during HCV infection. This might facilitate hepatocytic entry and reinfection of HCV . Micro RNA s (mi R s) play role in the regulation of gene expression. We aimed to characterize mi R expression profiles related to HCV infection and antiviral therapy in adult liver transplant recipients, with special emphasis on miRs predicted to target HCV receptors. Methods Twenty‐eight adult liver transplant recipients were enrolled in the study. Paired biopsies were obtained at the time of HCV recurrence and at the end of antiviral treatment. MiRs for HCV receptors were selected using target prediction software. Expression levels of mi R ‐21, mi R ‐23a mi R ‐34a, mi R ‐96, mi R ‐99a*, mi R ‐122, mi R ‐125b, mi R ‐181a‐2*, mi R ‐194, mi R ‐195, mi R ‐217, mi R ‐221, and mi R ‐224 were determined by reverse transcription–quantitative polymerase chain reaction. Results mi R ‐99a* and mi R ‐224 expressions were increased in HCV recurrence samples, while mi R ‐21 and mi R ‐194 were decreased in comparison to normal liver tissue. Increased expressions of mi R ‐221, mi R ‐224, and mi R ‐217 were observed in samples taken after antiviral therapy when compared with HCV recurrence samples. High HCV titer at recurrence was associated with higher level of mi R ‐122. Conclusions Samples at recurrence of HCV and after antiviral therapy revealed distinct HCV ‐related miR expression profiles, with significant dysregulation of those mi RNA s potentially targeting m RNA s of HCV receptors. In particular, mi R ‐194 and mi R ‐21 might be involved in the regulation of HCV receptor proteins' expression during HCV infection and antiviral therapy.