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N‐(3′, 4′‐dimethoxycinnamonyl) anthranilic acid alleviated experimental colitis by inhibiting autoimmune response and inducing CD 4 + CD 25 + regulatory T cells production
Author(s) -
Wen Quan,
Zhou Liping,
Chen Huan,
Zhang Yan
Publication year - 2013
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12266
Subject(s) - cytokine , medicine , colitis , lamina propria , immunology , mesenteric lymph nodes , inflammatory bowel disease , peripheral blood mononuclear cell , t cell , microbiology and biotechnology , immune system , pathology , chemistry , biology , biochemistry , disease , epithelium , in vitro
Background and Aim C rohn's disease treatments available today are not quite satisfactory. N‐(3′, 4′‐dimethoxycinnamonyl) anthranilic acid (3, 4‐ DAA ) has been proved to be effective in many autoimmune diseases. Therefore, we investigated the immunologic function of 3, 4‐ DAA on trinitrobenzene sulfonic acid ( TNBS ) colitis and human C rohn's disease. Methods Mice with TNBS ‐induced colitis were treated with 3, 4‐ DAA or 1‐methyl‐tryptophan (1‐ MT ). Colitis severity was assessed with clinical and histological scores. Cell proliferation, cytokine expression, and the percentage of CD 4 + CD 25 + T cells were measured in both mice and human samples. Results In mice treated with 3, 4‐ DAA , the clinical and histological scores were decreased ( P < 0.05); the proliferation of mesenteric lymph nodes ( MLN s) cells and lamina propria mononuclear cells ( LPMC s) were inhibited ( P < 0.05); T h1 cytokine expressions were decreased ( P < 0.05), and T h2 cytokine levels were increased ( P < 0.05). 3, 4‐ DAA also induced CD 4 + CD 25 + T cells expression (5.88 ± 2.1 vs 11.03 ± 2.93, P < 0.05) in mice MLN s. Transfer of these cells into TNBS colitis mice resulted in the reduction of the disease activity index ( DAI ) and histological scores. In LPMC s isolated from human C rohn's disease, 3, 4‐ DAA had the same effect. It can inhibit the cell proliferation, decrease T h1 cytokine expressions ( P < 0.05), and increase T h2 cytokine levels ( P < 0.05). The percentage of CD 4 + CD 25 + T cells were also increased (1.60 ± 0.14 vs 2.45 ± 0.50, P < 0.05). 1‐ MT treatment led to opposite outcomes. Conclusion 3, 4‐ DAA can alleviate the severity of colitis through inhibiting T h1 cells response, promoting T h2 cytokines expression and inducing CD 4 + CD 25 + T cells expression.