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Retracted : Gastroprotective effects of C orchorus olitorius leaf extract against ethanol‐induced gastric mucosal hemorrhagic lesions in rats
Author(s) -
Al Batran Rami,
AlBayaty Fouad,
Ameen Abdulla Mahmood,
Jamil AlObaidi Mazen M,
Hajrezaei Maryam,
Hassandarvish Pouya,
Fouad Mustafa,
Golbabapour Shahram,
Talaee Samaneh
Publication year - 2013
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12229
Subject(s) - medicine , ethanol , pharmacology , mucosal lesions , gastroenterology , biochemistry , biology
Background and Aim C orchorus olitorius is a medicinal plant traditionally utilized as an antifertility, anti‐convulsive, and purgative agent. This study aimed to evaluate the gastroprotective effect of an ethanolic extract of C . olitorius against ethanol‐induced gastric ulcers in adult S prague D awley rats. Methods The rats were divided into seven groups according to their pretreatment: an untreated control group, an ulcer control group, a reference control group (20 mg/kg omeprazole), and four experimental groups (50, 100, 200, or 400 mg/kg of extract). Carboxymethyl cellulose was the vehicle for the agents. Prior to the induction of gastric ulcers with absolute ethanol, the rats in each group were pretreated orally. An hour later, the rats were sacrificed, and gastric tissues were collected to evaluate the ulcers and to measure enzymatic activity. The tissues were subjected to histological and immunohistochemical evaluations. Results Compared with the extensive mucosal damage in the ulcer control group, gross evaluation revealed a marked protection of the gastric mucosa in the experimental groups, with significantly preserved gastric wall mucus. In these groups, superoxide dismutase and malondialdehyde levels were significantly increased ( P < 0.05) and reduced ( P < 0.05), respectively. In addition to the histologic analyses ( HE and periodic acid‐Schiff staining), immunohistochemistry confirmed the protection through the upregulation of H sp70 and the downregulation of B ax proteins. The gastroprotection of the experimental groups was comparable to that of the reference control medicine omeprazole. Conclusions Our study reports the gastroprotective property of an ethanolic extract of C . olitorius against ethanol‐induced gastric mucosal hemorrhagic lesions in rats.