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C rohn's disease and polymorphism of heat shock protein gene HSP 70‐2 in the C hinese population
Author(s) -
Chen Jun,
Ren Jianan,
Gu Guosheng,
Wang Gefei,
Wu Xiuwen,
Yan Dongsheng,
Liu Song,
Li Jieshou
Publication year - 2013
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12163
Subject(s) - odds ratio , genotype , psti , medicine , gastroenterology , confidence interval , polymorphism (computer science) , restriction fragment length polymorphism , population , allele , gene polymorphism , single nucleotide polymorphism , immunology , genetics , biology , gene , restriction enzyme , environmental health
Background and Aims C rohn's disease ( CD ) is a multifactorial disorder with a pivotal role of the genetic component. A single nucleotide polymorphism in heat shock protein 70‐2 ( HSP 70‐2 ) has been shown to be associated with a severe clinical course in CD . The purpose of this study was to identify associations between the HSP 70‐2 polymorphism and the clinical courses of CD in the C hinese population. Methods One hundred patients with CD and 190 healthy individuals were genotyped for the HSP 70‐2 PstI polymorphism by restriction fragment length polymorphism analysis. Results The genotype frequency of the PstI polymorphism did not differ between patients and controls. The A allele was higher in CD patients than in controls (61% vs 52%, P = 0.047, odds ratio [ OR] = 1.423, 95% confidence interval [ CI] : 1.004–2.015). Furthermore, this polymorphism was higher in the penetrating or fistula surgery of CD patients than in controls (63% vs 52%, P = 0.049, OR = 1.530, 95% CI : 1.001–2.337; T able [Table 4. Genotypes of Crohn's disease and perforation or fistula surgery]). But there was no significant difference between the penetrating or fistula surgery patients and no surgery patients ( P = 0.673, OR = 0.883, 95% CI : 0.495–1.574). We used multivariate analysis to determine the effects of genotypes on sex, disease behavior, disease location, and so on. No significant difference was observed between these parameters and genotype. Conclusion This study reported that the allele A of PstI polymorphism was the association between CD and HSP 70‐2 gene in the C hinese population. It was also association between penetrating or fistula surgery of CD and HSP 70‐2 gene in the C hinese population.