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Relationship between epithelial and stromal TRIM 28 expression predicts survival in colorectal cancer patients
Author(s) -
Fitzgerald Seán,
Sheehan Katherine M,
O'Grady Anthony,
Kenny Dermot,
O'Kennedy Richard,
Kay Elaine W,
Kijanka Gregor S
Publication year - 2013
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12157
Subject(s) - colorectal cancer , stromal cell , hazard ratio , medicine , trim , confidence interval , immunohistochemistry , tissue microarray , cancer research , cancer , carcinogenesis , proportional hazards model , oncology , pathology , computer science , operating system
Background and Aim TRIM 28 is a multi‐domain nuclear protein with pleotropic effects in both normal and tumor cells. In this study, TRIM 28 expression in epithelial and stromal tumor microenvironment and its prognostic role in colorectal cancer were investigated. Methods Immunohistological staining of TRIM 28 was evaluated in tissue microarrays constructed from 137 colorectal cancer patients. The correlations of TRIM 28 expression with clinicopathological features and p53 expression were studied. K aplan– M eier analysis and C ox proportional hazard modeling were used to assess overall survival ( OS ) and recurrence‐free survival ( RFS ). Results Strong epithelial TRIM 28 expression was found in 42% of colorectal cancer tissues. TRIM 28 expression correlated significantly with p53 expression in matched cases ( P = 0.0168, Spearman rank test). A high epithelial to stromal TRIM 28 expression ratio was associated with shorter OS ( P = 0.033; log‐rank test) and RFS ( P = 0.043; log‐rank test). Multivariate analysis showed that the epithelial to stromal TRIM 28 expression ratio was an independent predictor of OS (hazard ratio = 2.136; 95% confidence interval 1.015–4.498, P = 0.046) and RFS (hazard ratio = 2.100; confidence interval 1.052–4.191, P = 0.035). Conclusion A high TRIM 28 expression ratio between stromal and epithelial compartments in colorectal cancer tissue is an independent predictor of poor prognosis. The pathophysiological role of TRIM 28 in carcinogenesis may be dependent on expression levels and cell type within the tumor microenvironment.