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H elicobacter pylori cagA 12‐bp insertion can be a marker for duodenal ulcer in O kinawa, J apan
Author(s) -
Matsuo Yuichi,
Shiota Seiji,
Matsunari Osamu,
Suzuki Rumiko,
Watada Masahide,
Binh Tran Thanh,
Kinjo Nagisa,
Kinjo Fukunori,
Yamaoka Yoshio
Publication year - 2013
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/jgh.12067
Subject(s) - caga , polymerase chain reaction , primer (cosmetics) , helicobacter pylori , medicine , gastritis , microbiology and biotechnology , biology , gene , genetics , gastroenterology , virulence , chemistry , organic chemistry
Background and Aim H elicobacter pylori cagA can be classified into mainly two types ( E ast‐ A sian‐type and W estern‐type cagA ) according to the repeat regions located in the 3′ region. Recent studies showed that the W estern‐type cagA in strains from O kinawa, J apan formed a different cluster ( J ‐ W estern‐type cagA subtype). It has also been reported that J ‐ W estern‐type cagA possesses a 12‐bp insertion located in the 5′ region of cagA sequence. Methods The prevalence of 12‐bp insertion in cagA in O kinawa and the U nited S tates ( U . S .) was examined by DNA sequencing. The primer pair that can detect the 12‐bp insertion only by polymerase chain reaction was then designed. The prevalence of strains with 12‐bp insertion was examined in 336 strains isolated from O kinawa by polymerase chain reaction. Results In case of W estern‐type cagA / vacA s1m2 strains, the prevalence of 12‐bp insertion was significantly higher in strains isolated from O kinawa than that from the U . S . ( P = 0.002). Phylogenetic tree showed that strains with 12‐bp insertion formed two individual clusters within J ‐ W estern‐type cagA subtype; one is from O kinawa and another is from the U . S . The designed primer set showed high sensitivity (100%) and specificity (90.8%) in O kinawa. The 12‐bp insertion was found in 23.7%, 14.3%, 4.2%, and 4.0% of strains with duodenal ulcer ( DU ), gastritis, gastric cancer, and gastric ulcer ( GU ), respectively ( P < 0.001 for DU vs GU ) in O kinawa. Conclusions Although the mechanisms are unknown, the presence of 12‐bp insertion was associated with the presence of DU and might have a suppressive action on GU and gastric cancer.