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Rapid label‐free detection of Salmonella enterica with biolayer interferometry
Author(s) -
Zang ChengLin,
Zhang MingDi,
Zhang Yan,
Li YanSong,
Liu Kai,
Xie NanNan,
Sun ChunYan,
Zhang XiaoGuang
Publication year - 2021
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12896
Subject(s) - salmonella enterica , salmonella , foodborne pathogen , microbiology and biotechnology , detection limit , food safety , outbreak , pathogen , chemistry , virology , food science , biology , bacteria , chromatography , listeria monocytogenes , genetics
Abstract Salmonella enterica is an important foodborne pathogen. Its rapid and reliable detection in food is critical for the effective prevention of the outbreak of foodborne diseases. Here, we report a novel method for the rapid, specific, label‐free, and real‐time detection of S. enterica with biolayer interferometry (BLI) using an antibody as the receptor. The limit of detection for S. enterica in buffer was 1.6 × 10 5 CFU/ml at a binding time of 300 s. This method enabled the successful identification of S. enterica in complex food matrices (whole egg powder and beef samples) with satisfactory recovery rates. This BLI‐based method holds great promise in serving as a novel diagnostic tool in the field of food safety and other related fields for the rapid, label‐free, specific, and real‐time detection of S. enterica .