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A rapid detection of Escherichia coli O157 : H7 by competition visual antigen macroarray
Author(s) -
Xia Junfang,
Qiu Shi,
Zeng Haijuan,
Liu Cheng,
Liu Qing
Publication year - 2021
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12872
Subject(s) - escherichia coli , antigen , microbiology and biotechnology , foodborne pathogen , antibody , chemistry , contamination , bacteria , biology , biochemistry , listeria monocytogenes , gene , immunology , ecology , genetics
Pathogenic bacterial contamination is a serious problem for the food industry and in public health. Rapid, accurate and affordable testing for pathogenic bacterial strains is desirable. In this study, a competition visual antigen macroarray (CVAM) for rapid detection of Escherichia coli O157:H7 ( E. coli O157:H7) has been developed. This array was able to utilize an HRP‐labeled anti‐ E. coli O157:H7 MAb at a concentration of 1:20000 while having a similar sensitivity of 10 5 CFU/ml for E. coli O157:H7 detection as double antibody sandwich array and conventional ELISA. The detection time of CVAM was short as 2 h and can be examined directly by the naked eye. Moreover, there was no cross‐reactivity in the detection of tested in this study. Application of CVAM for the detection of E. coli O157:H7 artificially contaminated milk samples was feasible that showed the antigen array could be applied in the detection of food pathogen infections.