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SSEL , a selective enrichment broth for simultaneous growth of Salmonella enterica , Staphylococcus aureus , Escherichia coli O157 : H7 , and Listeria monocytogenes
Author(s) -
Qu Yang,
Bai Yalong,
Liu Yanhong,
Zhou Changyan,
Zhou Xiujuan,
Zhang Donglai,
Shi Chunlei,
Suo Yujuan
Publication year - 2020
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12837
Subject(s) - listeria monocytogenes , salmonella enterica , microbiology and biotechnology , escherichia coli , salmonella , staphylococcus aureus , chemistry , bacteria , nalidixic acid , biology , food science , antibiotics , antibiotic resistance , biochemistry , genetics , gene
Salmonella enterica , Staphylococcus aureus , Escherichia coli O157: H7, and Listeria monocytogenes may contaminate similar types of food and cause foodborne disease. The objective of this study was to develop a selective enrichment broth for simultaneous enrichment of Salmonella enterica , Staphylococcus aureus , Escherichia coli O157: H7, and Listeria monocytogenes (SSEL) using nalidixic acid, acriflavine, lithium chloride, and sodium cholate as selective agents. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37°C with 10–100 CFU/mL of inoculation concentration, but also could successfully support the simultaneous enrichment of target pathogens with similar growth rates and inhibit the growth of most nontarget bacteria effectively. The enrichment effect of SSEL was confirmed by artificial contamination test coupled with multiplex PCR. In summary, SSEL has been shown to be a promising multiplex selective enrichment broth for the detection of the four pathogens on a single‐assay platform.