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Determination of extended spectrum β‐lactamase producing Enterobacteriaceae from cheese samples sold in public bazaars
Author(s) -
Husan Omar,
Çadirci Özgür
Publication year - 2019
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12680
Subject(s) - klebsiella oxytoca , enterobacter cloacae , enterobacter aerogenes , enterobacteriaceae , klebsiella pneumoniae , citrobacter , microbiology and biotechnology , klebsiella , biology , salmonella , enterobacter , citrobacter freundii , veterinary medicine , escherichia coli , bacteria , gene , medicine , genetics
In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive isolates were obtained from 34 samples (26.6%). Identification of 148 isolates were as follows; 79 Escherichia coli (54.5%), 39 Klebsiella pneumoniae (26.3%), 16 Klebsiella oxytoca (10.8%), 5 Citrobacter youngae (3.4%), 4 Shigella bodii (2.7%), 2 Klebsiella ozaenae (1.53%), 2 Enterobacter cloacae (1.53%), and 1 Enterobacter aerogenes (0.67%). Polymerase chain reaction analysis indicated that 64 isolates carried the bla CTX‐M gene (43.2%), while 39 isolates carried the bla TEM gene (26.3%), and 16 isolates carried the bla SHV gene (10.8%). It was concluded that cheeses sold in local markets are an important source of enteric bacteria that can produce ESBL. Therefore, cheeses can be considered as a potential source for spread of ESBL genes from foods to human. Practical Applications Enterobacteriaceae strains producing ESBL have been isolated from many foods, especially foods of animal origin, form a potential risk to public health due to zoonotic character.