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Virulence genes and antibiotic resistance of Salmonella recovered from a wet market in Thailand
Author(s) -
Sripaurya Bussara,
Ngasaman Ruttayaporn,
Benjakul Soottawat,
Vongkamjan Kitiya
Publication year - 2019
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12601
Subject(s) - salmonella , virulence , tetracycline , antibiotic resistance , biology , contamination , multiple drug resistance , veterinary medicine , microbiology and biotechnology , pathogen , food science , drug resistance , antibiotics , bacteria , gene , medicine , ecology , biochemistry , genetics
Salmonella is an important foodborne pathogen, which is often found on chicken meat and various sites in wet markets in Southeast Asia, thus requiring close monitoring for effective control measures. In this study, 130 samples from chicken meat, environment, and wastewater from three chicken meat stalls at a wet market were tested for Salmonella spp. For stall A, B, and C, 20 (44.4%), 8 (17.4%), and 9 (23.1%) samples were positive for Salmonella , respectively. In this study, Salmonella was often detected on working tables, scales, cutting board, knives, and bucket, suggesting important food contact equipment that typically links to the presence of Salmonella at local stalls or retails. This equipment should be emphasized for the improved control measures to minimize Salmonella contamination. Using the nine virulence genes ( sip B, prg H, spa N, org A, tol C, sit C, sif A, cdt B, and sop B, pattern IV ( sif A and cdt B negative) was a predominant pattern. Most Salmonella isolates ( n = 42) were resistant to tetracycline, but none showed resistance to ceftriaxone. Data suggest potential sources of Salmonella , including chicken and several environmental sites at a wet market. This could be of a public health concern as multiple patterns of virulence genes and multidrug resistance were observed. Practical applications Salmonella spp. are commonly found on chicken meat sold at a retail level and, commonly in Southeast Asia, at wet markets where chicken carcasses are processed. This could be a typical source of Salmonella contamination, thus requiring close monitoring for effective control measures. Information obtained from this study reported the distribution and characteristics regarding the presence of virulence genes and antibiotic resistance. This will help effective implementation for ensuring food safety at the wet markets. This is also a useful tool for market management to further minimize the spread of some Salmonella isolates shown to be multidrug resistance that is found in chicken and several environmental sites at a wet market.