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Prevalence, genotyping, serotyping, and antibiotic resistance of isolated Salmonella strains from industrial and local eggs in Iran
Author(s) -
Karimiazar Farshad,
Soltanpour Mohammad S.,
Aminzare Majid,
Hassanzadazar Hassan
Publication year - 2019
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12585
Subject(s) - salmonella , salmonella enterica , serotype , biology , genotyping , microbiology and biotechnology , antibiotic resistance , nalidixic acid , antimicrobial , salmonella enteritidis , veterinary medicine , genotype , antibiotics , gene , bacteria , genetics , medicine
This present study aimed to evaluate prevalence and characterization of serotypes, antimicrobial resistance (AMR) and genotypic profiles of Salmonella isolates in industrial and local eggs from Zanjan province of Iran. A total of 120 egg samples were collected and processed according to bacteriological analytical manual to isolate Salmonella strains from both eggs surface and contents. PCR method and genotyping were used to verify, identification and classification of isolates by screening inv A genes and 16 s‐rRNA gene sequencing. Salmonella contamination rate in eggshell and contents of industrial and local eggs were 0% and 1.66%, respectively. High degree of AMR was observed to nalidixic acid and erythromycin. Gene sequencing for each isolates showed more similarity with Salmonella enterica subsp. enterica serovar Enteritidis and Salmonella enterica subsp. enterica serovar Typhimurium strains. Practical applications Salmonella contamination in eggs and the role of healthy chickens as reservoir and distributer of Salmonella in poultry farms can be a common food safety concern for public health. Therefore, Continuous monitoring of presence Salmonella and their antimicrobial resistance is of critical importance. Present study gives an insight of the current statues of egg's contamination with Salmonella isolates, their antimicrobial resistance, genotupic profile, and epidemiological relationship in Iran.