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Safety evaluation of subchronic feeding of nisI transformed Lactobacillus plantarum in Sprague‐Dawley rats
Author(s) -
Liu Haiyan,
He Xiaoyun,
Xu Wentao,
Huang Kunlun,
Zhang Jianwei
Publication year - 2018
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12427
Subject(s) - lactobacillus plantarum , hematology , adverse effect , pathological , body weight , medicine , biology , physiology , endocrinology , zoology , lactic acid , bacteria , genetics
The nisI gene was inserted into Lp ( Lactobacillus plantarum ) genome and obtained Lp590 ( L . plantarum 590). The nisI gene can help Lp590 tolerate higher concentration of nisin. Although Lp was generally recognized as safe, the safety of the nisI transformed Lp is unknown. In this study, six groups of Sprague‐Dawley (three groups/sex) rats were orally administered with Lp, Lp590 or physiological saline for 90 days. Nutritional status and growth performance were evaluated followed by analysis of blood biochemistry, hematology and organ variables. Compared with control, we did not find any treatment‐related adverse or toxic effects on the examination of clinical signs, body weight, organ weight, serum biochemistry, hematology, gross, and microscopic anatomic pathological examination. These results demonstrated that the Lp590 is as safe as Lp in our subchronic feeding trial. Practical applications The purpose of this study is to evaluate the safety of transformed Lactobacillus plantarum . In this current 90‐day feeding study, body weight, feed consumption and toxicological response variables (hematological parameters, serum chemistry, absolute, and relative organ weight) were measured, and gross as well as microscopic pathology were examined. No adverse effects were observed on animals. The results indicated that Lp590 is as safe as Lp. The result can add the safety evidence of transformed microorganism.