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Molecular Characterization of Gyr‐A Gene Polymorphism in Salmonella Enterica Serovar Enteritidis Isolated of Egg Shells
Author(s) -
Doosti Abbas,
MokhtariFarsani Abbas,
Chehelgerdi Mohammad
Publication year - 2016
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12276
Subject(s) - biology , salmonella enteritidis , salmonella enterica , serotype , microbiology and biotechnology , restriction enzyme , restriction fragment length polymorphism , gene , salmonella , genomic dna , genetics , polymerase chain reaction , bacteria
Salmonella is one of the important bacteria in egg and as major source in bacterial infection transfer to human and animals. It has been proven that gyrA gene can mediate in resistance and reduced susceptibility to fluoroquinolones, therefore the aim of the present study was to determine gyrA gene polymorphism in S. enterica ser. Enteritidis isolated from egg shell using PCR‐RFLP assay. A total of 300 samples of egg shells were collected from seven aviculture in Chaharmahal Va Bakhtiari province and genomic DNA was extracted using DNA extraction kit. PCR‐RFLP assay was used to identification of gyrA gene polymorphism using HinfI and BanI restriction endonuclease enzymes. A total of 85 ser. Enteritidis was detected from 300 samples and gyrA gene was isolated from all 85 positive ser. Enteritidis that out of 85 gyrA gene, 23 samples (27%) and 25 samples (29.4%) were respectively positive for HinfI and BanI enzymes. The results of the present study showed a relatively low prevalence of ser. Enteritidis infection in egg shells, but the rate of gyrA gene mutation was high that is very disturbing. Practical Applications Currently, at least 2610 serovars of S.enterica have been recognized worldwide, and all seem to be capable of causing disease in humans. Therefore, S.enterica remains a microbial threat in terms of standards and food safety, and the use of molecular techniques for analysis of antimicrobial resistance and its reasons in Salmonella spp. specifically S.enterica is of high importance. Our study may provide more insights to design new therapeutic plans including limited use of antimicrobial drugs in human and animals, performing antimicrobial susceptibility tests to select suitable antimicrobial agent, and application of recommended dosage of antibiotic. Also the present study can be used to help guide selection of antimicrobials for use in poultry industry and the treatment of diseases associated with Salmonella spp. especially S. enterica subsp enterica ser. Enteritidis.