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Antimicrobial Activity of Roselle Hibiscus Sabdariffa Calyx Extracts on Culture Media and Carrots Against Multidrug‐Resistant Salmonella Strains Isolated from Raw Carrots
Author(s) -
GutiérrezAlcántara Eduardo J.,
GómezAldapa Carlos A.,
RománGutiérrez Alma D.,
RangelVargas Esmeralda,
GonzálezOlivares Luis G.,
CastroRosas Javier
Publication year - 2016
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12259
Subject(s) - hibiscus sabdariffa , calyx , salmonella , antimicrobial , sodium hypochlorite , chemistry , hibiscus , food science , multiple drug resistance , acetic acid , microbiology and biotechnology , antibiotics , bacteria , biology , horticulture , biochemistry , genetics , organic chemistry
Presence of antibiotic‐resistant Salmonella strains on raw carrots was evaluated, as was the antibacterial activity of roselle calyx extracts against any identified resistant Salmonella strains. One hundred samples of carrots were collected from markets in Pachuca, Mexico. Salmonella was isolated from the carrot samples by culture procedure. Susceptibilities to sixteen antibiotics were determined for the isolated Salmonella strains by standard test. Antibacterial activity versus the isolated strains was measured for roselle extracts produced with water, methanol, acetone and ethyl acetate. Finally, the antibacterial effect of roselle extracts, sodium hypochlorite, acetic acid and colloidal silver against Salmonella strains isolated from carrots was evaluated on contaminated carrots. Thirteen Salmonella strains were isolated from 11% of the carrot samples. All strains exhibited resistance to at least two of the tested antibiotics. Roselle extracts caused a greater reduction in concentration of Salmonella than the sodium hypochlorite, acetic acid and colloidal silver on contaminated carrots. Practical Applications Roselle Hibiscus sabdariffa calyx extracts exhibited antimicrobial activity on culture media and on carrots against multidrug‐resistant Salmonella strains. The information presented shows that water extract of roselle calyx may be a potentially useful addition to disinfection procedures of raw carrots in field, carrot processor plants, restaurants and home.