Premium
Rapid Identification of P seudomonas aeruginosa and P seudomonas fluorescens Using R aman Spectroscopy
Author(s) -
Yilmaz Ayse Gamze,
Temiz Havva Tumay,
Acar Soykut Esra,
Halkman Kadir,
Boyaci Ismail Hakki
Publication year - 2015
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12200
Subject(s) - principal component analysis , raman spectroscopy , identification (biology) , spectroscopy , pathogen , biology , chemistry , computational biology , microbiology and biotechnology , mathematics , ecology , physics , statistics , optics , quantum mechanics
Rapid and accurate identification of P seudomonas species, especially in drinking water, is of great importance for human health. In the present study, we aim to develop a fast and reliable verification method, which does not require intensive microbiological procedures. In this method, specific R aman spectra of P seudomonas aeruginosa , P seudomonas fluorescens and B urkholderia pseudomallei were collected directly from the medium and principal component analysis was used for the data mining process of collected R aman spectra. Application of R aman spectroscopy accompanied with principal component analysis enabled us to differentiate the two most dominant accompanying flora among pathogen P seudomonas species. Based on R aman spectroscopy, an alternative cultivation‐free verification method with 15 min analysis time was developed. It is envisioned that using this method will also contribute to prevent the unnecessary closure of water wells. Practical Applications It is well known that P seudomonas sp. is commonly found in water and their detection is significant in terms of human health. In the present study, a rapid and cultivation‐free verification method has been developed for the identification and differentiation of P seudomonas sp. namely, P seudomonas aeruginosa , P seudomonas fluorescens and B urkholderia pseudomallei . The results of the study offer that R aman spectroscopy coupled with principal component analysis seems to be a simple alternative verification method with a shortened analysis time as low as 15 min.