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In vitro Anti‐Adherent Assessment of Selected Lactic Acid Bacteria Isolates Against S almonella Typhimurium and L isteria monocytogenes to Caco‐2 Cells
Author(s) -
Sribuathong Supannikar,
Saengprakai Janpen,
Trevanich Sudsai
Publication year - 2014
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12123
Subject(s) - microbiology and biotechnology , probiotic , lactic acid , bacteria , in vitro , pathogen , lactobacillus plantarum , pathogenic bacteria , biology , fermentation , strain (injury) , food science , chemistry , biochemistry , genetics , anatomy
The present study aimed to evaluate in vitro anti‐adherence abilities of the probiotic potential of three selected lactic acid bacteria ( LAB ) strains isolated from fermented broken rice with two foodborne pathogenic bacteria to C aco‐2 cells. L actobacillus plantarum Ban Pra Doke 110 was the most adherent strain with an adherence of 17.66% at a concentration of added bacterial cells of 2.0 × 10 8 cfu/mL (multiplicities of infection 100). In the exclusion, competition and displacement tests, L act. plantarum PD 110 showed the most anti‐adherence abilities against S almonella T yphimurium A merican T ype C ulture C ollection 13311 and L isteria monocytogenes S cottA to C aco‐2 cells. Moreover, the presence of its spent culture supernatant exhibited the highest decrease in the percentage numbers of the adherent pathogenic bacteria used in all tests. The effectiveness of each LAB strain was tested to adhere to C aco‐2 cells or to prevent the adherence of the test pathogenic bacteria varied widely depending on the genus and species, source of origin, and assay condition, as well as adherent‐promoting factors. Practical Applications Foodborne pathogen infection to the human intestinal epithelial surface cells is a critical step for the survival and cause foodborne illness. The anti‐adherent assessment of novel probiotic is of importance for the inhibition of pathogen infections. L actobacillus plantarum PD 110 isolated from fermented broken rice could be one of these good candidates. The demonstrated in vitro anti‐adherent activity of L act. plantarum PD 110 to Caco‐2 cells recommends its possible application as a probiotic candidates in order to inhibit the adherence of S almonella T yphimurium and L isteria monocytogenes to the human gastrointestinal tract. Therefore, this strain can be considered as a novel potential starter culture to improve the quality and safety of functional foods.