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An Analysis of E scherichia coli Isolations for Antimicrobial Resistance Genes
Author(s) -
Colobătiu Liora,
Oniga Ovidiu,
Tăbăran Alexandra,
Mihaiu Romolica,
Mirel Simona,
Daniel Sorin Dan,
Mihaiu Marian
Publication year - 2014
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12118
Subject(s) - antimicrobial , tetracycline , antibiotic resistance , raw meat , biology , microbiology and biotechnology , ampicillin , sulfamethoxazole , escherichia coli , gene , food science , antibiotics , genetics
This research was conducted in order to reveal antimicrobial resistance patterns and the prevalence of genes encoding resistance in E scherichia coli isolated from raw poultry meat. One hundred samples of poultry meat were collected, and 51 isolates of E . coli were recovered and further tested for antimicrobial susceptibility to 11 antimicrobials, using the classical disk diffusion method. Fifteen isolates, showing a high degree of resistance among the collection were examined for antimicrobial resistance genes by multiplex polymerase chain reaction. Resistance was most frequently observed to tetracycline (84.26%), ampicillin (73.03%) and sulfamethoxazole (66.29%). The tetracycline resistance genes TetA and TetB and beta‐lactam TEM gene were frequently detected in the tested isolates. Genes responsible for resistance to sulfonamides (33.33%) and trimethoprim (26.66%) were also found.E . coli isolates recovered from raw poultry meat are highly resistant to antimicrobials and carry antimicrobial resistance genes that could be transferred to other microbes in the food chain, even important human pathogens. Practical Applications The findings from this work provide baseline data for further antimicrobial resistance studies especially in our country, were antimicrobial resistance epidemiology needs constant improvement. To our knowledge, this is one of the first reports regarding the identification of various resistance genes identified in E . coli meat isolations in R omania. Moreover, the work contributes to the risk assessment concerning the possible E . coli threat in food products and draws a warning signal for further actions that can assist the implementation of efficient measures to prevent possible adverse public health consequences.