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Molecular Typing of C ronobacter Strains from Food in C hina by Enterobacterial Repetitive Intergenic Consensus Sequence PCR ( ERIC ‐ PCR ) and Sequence Analysis of the gyrB Gene
Author(s) -
Chen Wanyi,
Ai Lianzhong,
Yang Jielin,
Ren Jing,
Li Yunfei,
Guo Benheng
Publication year - 2013
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12068
Subject(s) - typing , intergenic region , subtyping , biology , polymerase chain reaction , sequence analysis , phylogenetic tree , genetics , multilocus sequence typing , gene , virulence , microbiology and biotechnology , genotype , genome , computer science , programming language
The occurrence of outbreaks of C ronobacter strains causing necrotizing meningitis in C hina highlights the need for strain characterization and subtyping of this pathogenic species. A total of 43 C ronobacter isolates and five E nterobacteriaceae strains were used in this study. The strains were characterized using two molecular typing methods, including enterobacterial repetitive intergenic consensus sequence polymerase chain reaction ( ERIC ‐ PCR ) and sequence analysis of the gyrB gene. Genetic profiles of cluster analysis from these two molecular typing tests clearly showed that there was no close relationship between C ronobacter spp. and E nterobacteriaceae strains. Discriminatory index of ERIC ‐ PCR typing for the 43 C ronobacter isolates and five E nterobacteriaceae strains was high ( D = 0.984) by S impson's index of diversity based on the similarity value of more than 80% using B io N umerics version 5.0. Sequence analysis of the gyrB gene was proven to be suitable for the phylogenetic analysis of the C ronobacter strains. These results demonstrate that ERIC ‐ PCR combined with sequence analysis of the gyrB gene may be a reliable, rapid typing strategy for C ronobacter strains. Practical Applications Molecular typing of foodborne pathogenic bacteria has been shown as a useful tool for tracking the source of infection and detection of virulent strains, as well as for determining the geographical and host distribution of possible variants. ERIC ‐ PCR provides rapid clustered results when a large number of C ronobacter spp. isolates need to be subtyped rapidly. Furthermore, partial sequence analysis of the gyrB gene was utilized to differentiate among the closely related isolates. The results of the current study suggest that ERIC ‐ PCR in conjunction with the gyrB sequence analysis would provide a reliable and accurate typing strategy for C ronobacter spp. strains. This combination of techniques can be used as a rapid means of comparing C ronobacter spp. isolates for epidemiological investigations.