Premium
Detection of B rucella spp. in Stretched Curd Cheese as Assessed by Molecular Assays
Author(s) -
Capuano Federico,
Capparelli Rosanna,
Mancusi Andrea,
Esposito Salvatore,
Corrado Federica,
Guarino Achille
Publication year - 2013
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12034
Subject(s) - herd , food science , brucellosis , cow milk , biology , contamination , sheep milk , veterinary medicine , zoology , medicine , immunology , ecology
The study concerns the presence of B rucella spp. in the stretched curd cheese produced in an area characterized by the presence of a relevant number of herds infected with B rucella spp. One hundred ninety‐one cheese samples made with milk from different species were tested by two molecular assays. Positive samples were further submitted to sequence analysis of the IS 711 gene. Eleven out of 98 of the samples made with buffalo milk alone resulted contaminated. The prevalence in cheese samples from cow milk alone and milk from cow and buffalo was 2.7% and 10.5%, respectively. No sample was positive to the microbiological O ffice I nternational des E pizooties detection tests of B rucella spp. Practical Application The aim of the study is to evaluate the use of molecular assays as a tool to support microbiological and immunological tests carried out to eradicate brucellosis. Molecular assays shows to be a useful aid for the monitoring of herds health and the safety of dairy products evaluation; furthermore, their application to dairy products allows the implementation of the procedures to trace those infected farms that supply milk to dairies.