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Anti‐listerial and Anti‐biofilm Activities of Potential Probiotic L actobacillus Strains Isolated from T unisian Traditional Fermented Food
Author(s) -
Ben Slama Rihab,
Kouidhi Bochra,
Zmantar Tarek,
Chaieb Kamel,
Bakhrouf Amina
Publication year - 2013
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/jfs.12017
Subject(s) - listeria monocytogenes , biofilm , lactic acid , microbiology and biotechnology , chemistry , bacteria , probiotic , food science , lactobacillus plantarum , fermentation , biology , genetics
Three L actobacilli were isolated from traditional T unisian fermented food and then characterized for its ability to inhibit L isteria monocytogenes growth. Antagonistic effect of L actobacillus plantarum on four L . monocytogenes strains was tested in soft artisanal cheese. Anti‐biofilm activity of L actobacillus extracts was also tested. Our results demonstrate that the selected lactic acid bacteria ( LAB ) extract exhibited a good antibacterial effect against L . monocytogenes ATCC 19115 with low MIC s values: 8.33 ± 2.1, 20 ± 1.2 and 23.33 ± 1, respectively. L . plantarum also exhibited a stronger inhibitory effect against L . monocytogenes when grown in cheese. Moreover, a potential anti‐biofilm effect of the three LAB extracts with BIC 50 values ranging from 5% to 15% for L . monocytogenes ATCC 19115 was demonstrated. Although LAB extracts were able to eradicate significantly a preformed L . monocytogenes biofilm ( P < 0.05). Growth inhibition of preformed biofilm was more difficult to achieve. LAB could be used as a bioprotective culture in cheese ripening to prevent L isteria growth. Practical Applications A wide range of lactic acid bacteria ( LAB ) produce bacteriocins, which were essentially active against the food‐borne pathogen L isteria monocytogenes. 2, 3‐bis (2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐5‐[(phenylamino) carbonyl]‐2 H ‐tetrazolium hydroxide ( XTT ) reduction method was used to assess the anti‐biofilm and anti‐listerial activity of LAB extract. XTT reduction assay is dependent on the microbial respiratory activity. Since XTT is used for quantification of the microbial respiratory activity, it was used to quantify viable mature biofilm and to compare LAB activity against planktonic and biofilm cells.