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Optimizing the liquid‐state fermentation conditions used to prepare a new Shan‐Zha‐Ge‐Gen formula‐derived probiotic
Author(s) -
Liu Fuyu,
Song Min,
Wang Xinke,
Sun Yizheng,
Liu Xiaoyun,
Zhou Fengqin,
Guo Qingmei
Publication year - 2021
Publication title -
journal of food processing and preservation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.511
H-Index - 48
eISSN - 1745-4549
pISSN - 0145-8892
DOI - 10.1111/jfpp.15699
Subject(s) - fermentation , probiotic , food science , chemistry , ascorbic acid , antioxidant , gallic acid , lactobacillus plantarum , rutin , polyphenol , lactic acid , biochemistry , biology , bacteria , genetics
Abstract In this study, we aimed to develop a new Shan‐Zha‐Ge‐Gen formula‐derived probiotic by optimizing the parameters of the liquid‐state fermentation method. Optimized fermentation parameters were derived using principal component analysis. We investigated the effects of the fermentation time, fermentation strain, material‐to‐liquid ratio, and carbon content on the active components and antioxidant activity (AA) of the fermented Shan‐Zha‐Ge‐Gen formula. The following optimal fermentation conditions were identified in our study: material‐to‐liquid ratio, 1:10 (M/V); fermentation time, 72 hr; carbon content, 17%; and fermentation strain, Lactobacillus plantarum ACBC271. Under these optimal conditions, the total flavonoid content, total phenolic content, and puerarin content of the fermented probiotic were 77.04 ± 0.30‐mg rutin equivalents/g sample, 33.86 ± 0.49‐mg gallic acid equivalents/g sample, and 5.29 ± 0.15‐mg/g sample, respectively. The AA of the formula was assessed on the basis of its 1,1‐diphenyl‐2‐pricrylhydrazyl and hydroxyl‐scavenging ability and ferric‐reducing antioxidant power, which were 16.69 ± 0.20‐mg ascorbic acid/g sample, 35.93 ± 0.06‐mg ascorbic acid/g sample, and 0.02 ± 0.00‐mmol Fe 2+ /g sample, respectively. Liquid‐state fermentation significantly increased the concentrations of the active components and the AA of the probiotic. Collectively, our findings indicate that fermented Shan‐Zha‐Ge‐Gen formula‐derived probiotic can serve as a food source for polyphenols with high antioxidant potential. Novelty impact statement We developed a new probiotic, Shan‐Zha‐Ge‐Gen formula‐derived food, using raw materials from traditional Chinese medicine. The fermented Shan‐Zha‐Ge‐Gen had increased concentrations of active components and antioxidant activities compared with the traditional Shan‐Zha‐Ge‐Gen formula, which could provide several health benefits to patients with cardiovascular diseases.