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Effects of temperature on the denaturation and aggregation of ( Lateolabrax japonicus ) myosin from sea bass surimi
Author(s) -
Liu Fangfang,
Huang Hui,
Lin Wanling,
Li Laihao,
Wu Yanyan,
Yang Shaoling,
Yang Xianqing,
Wang Yueqi
Publication year - 2021
Publication title -
journal of food processing and preservation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.511
H-Index - 48
eISSN - 1745-4549
pISSN - 0145-8892
DOI - 10.1111/jfpp.15417
Subject(s) - lateolabrax , sea bass , myosin , chemistry , denaturation (fissile materials) , bass (fish) , fishery , food science , biochemistry , biology , fish <actinopterygii> , nuclear chemistry
This study aimed to investigate the effects of temperatures of 30–90°C on myosin denaturation and aggregation in sea bass ( Lateolabrax japonicus ) during setting. The result indicated that the myosin of sea bass began to form gels at 60°C, and surface hydrophobicity increased by around 8.9% at 40°C and reached its maximun at 60°C. During setting at 40°C and 60°C, the ratios of α‐helix of myosin reduced gradually and finally decreasing by 17.1% and 25.2%, respectively. Incubating at 90°C, α‐helix continued to transform into random coil. Association with the results of surface morphology, it further confirmed that the temperatures of 40°C and 60°C were two important denaturation and aggregation temperatures. At 90°C, myosin of sea bass formed a fine and dense gel network under the joint action of nondisulfide covalent bond, disulfide bond and hydrophobic interaction. Practical applications The raw materials for marine surimi are becoming scarcer with marine overfishing and the increasing demand for surimi. Sea bass, an important economic mariculture fish in China, has white meat and less bone spur and is advantageous for the development of surimi products as raw materials. During heating of surimi, the change of myosin structure is a main important key to forming a gel network. This research revealed the changes of sea bass myosin from denaturation, aggregation to gel formation during heating and provided a theoretical basis for the processing of sea bass surimi.