Premium
Recovery of major phenolic acids and antioxidant activity of highland barley brewer’s spent grains extracts
Author(s) -
Ikram Sana,
Zhang Huijuan,
Ming Haoyi,
Wang Jing
Publication year - 2020
Publication title -
journal of food processing and preservation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.511
H-Index - 48
eISSN - 1745-4549
pISSN - 0145-8892
DOI - 10.1111/jfpp.14308
Subject(s) - chemistry , antioxidant , dpph , abts , food science , ferulic acid , caffeic acid , acetone , coumaric acid , extraction (chemistry) , biochemistry , chromatography
Highland barley brewer's spent grain (BSG) is a valuable by‐product with a significant amount of antioxidant phenolic acids (APA). The aim of the present study was to assess the total phenolic contents and the radical scavenging activity of highland barley BSG. The in vitro methods, such as 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulphonic acid) ABTS radical scavenging activity assay, ferric reducing/antioxidant power assay, 2‐deoxyribose assays, and hydrogen peroxide scavenging activity, were applied. The extracts produced with acetone from highland barley BSG exhibited antioxidant activities. However, at 40% v/v extract exhibited the highest antioxidant capacity and the highest content of total phenolic acids. HPLC‐DAD analysis also demonstrated that the highland barley BSG extracts generated with 40% v/v exhibited the highest amount of major phenolic acids such as ferulic acid (5.07 mg/g) followed by p ‐coumaric acid (2.10 mg/g) and then caffeic acid (0.644 mg/g). The results indicated the highland barley BSG was a valuable source of antioxidant phenolic compounds. Whereas the solid–liquid extraction technique with 40% v/v acetone was as simple, low cost, and most effective method to recover APA from highland barley BSG. Practical applications Highland barley BSG is attracting increasing attention due to its significant potential as a natural antioxidant to reduce the risk of diseases and to promote health.