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Preparation of high purity triacylglycerol of punicic acid from pomegranate seeds
Author(s) -
Pamisetty Aruna,
Rangaswamy Manjunatha Javagal,
Singh Ravendra Pratap
Publication year - 2018
Publication title -
journal of food processing and preservation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.511
H-Index - 48
eISSN - 1745-4549
pISSN - 0145-8892
DOI - 10.1111/jfpp.13647
Subject(s) - chemistry , chromatography , high performance liquid chromatography , fatty acid , fraction (chemistry) , sample preparation , food science , biochemistry
Pomegranate seeds are rich in punicic acid, a ω‐5 fatty acid which renders highly beneficial effects on human health. However, due to very unstable nature of punicic acid, its quantification becomes difficult. In the present study, we propose a simple methodology of preparation of a sample of Triacylglycerol of punicic acid (Pu‐Pu‐Pu, > 97% purity) from pomegranate seeds using RP‐HPLC. Fraction 6 from HPLC of PSO was found to possess a high content of Pu. Further identification and characterization of the sample have been carried out using GC‐MS and NMR. The sample has been found to be stable for 90 days at −20 °C. Practical applications The quantification of Pu is difficult due to its unstable nature and non‐availability of Pu standard. However, owing to it stability, the sample of the TAG of Pu (Pu‐Pu‐Pu, > 97% purity) prepared from PSO could be satisfactorily used as internal standard for the quantification of Pu in PSO. This sample of the TAG of Pu could be applied in characterizing PSO based on PU content in future research.