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A Continuous Fractionation of Ginsenosides and Polysaccharides From Panax ginseng Using Supercritical Carbon Dioxide Technology
Author(s) -
Yu ILung,
Yu ZerRan,
Koo Malcolm,
Wang BeJen
Publication year - 2016
Publication title -
journal of food processing and preservation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.511
H-Index - 48
eISSN - 1745-4549
pISSN - 0145-8892
DOI - 10.1111/jfpp.12655
Subject(s) - ginseng , chemistry , fractionation , polysaccharide , supercritical fluid , supercritical carbon dioxide , chromatography , extraction (chemistry) , ethanol , fraction (chemistry) , araliaceae , solvent , carbon dioxide , supercritical fluid extraction , biochemistry , organic chemistry , alternative medicine , pathology , medicine
A continuous supercritical carbon dioxide ( SC‐CO 2 ) fractionation was conducted to obtain ginsenosides and polysaccharides from P anax ginseng extract. P . ginseng was mixed with ethanol at a ratio of 1:4 (volume by weight) for 24 h to yield ethanol extract. SC‐CO 2 fractionation was performed using an orthogonal design to obtain optimal fractionation parameters. The conditions were: three different temperatures (40, 50 and 60C), three different pressure levels (10, 20 and 30 MPa ), three flow rates of SC ‐ CO 2 (2, 4 and 6 mL/min) and three flow rates of ginseng extract liquor (1, 2 and 3 mL/min). The highest concentration of total ginsenosides was obtained in the fraction ( F ) whereas the highest concentration of total polysaccharides was retained in the residual. The highest selectivity of ginsenosides and polysaccharides were obtained at 60C and 30 MPa . The greatest free radicals scavenging ability and antioxidant ability were obtained in F . Practical Applications Ginsenosides and polysaccharides from P anax ginseng have been postulated to contribute a number of positive health effects. Traditional extraction techniques are associated with the disadvantages of solvent residues and its associated toxicity. In the present study, a continuous preparation method using supercritical fluid technology was described with the optimal temperature and pressure for the separation of P . ginseng ginsenosides and polysaccharide. Our findings can be served as a basis to facilitate future scale‐up industrial applications.