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Pretreatment of sugarcane bagasse by OX‐B to enhancing the enzymatic hydrolysis for ethanol fermentation
Author(s) -
da Siva Martins Luiza Helena,
Komesu Andrea,
Moreira Neto João,
Oliveira Johnatt Allan Rocha,
Rabelo Sarita Cândida,
Costa Aline Carvalho
Publication year - 2021
Publication title -
journal of food process engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 45
eISSN - 1745-4530
pISSN - 0145-8876
DOI - 10.1111/jfpe.13579
Subject(s) - bagasse , chemistry , ethanol fuel , enzymatic hydrolysis , pulp and paper industry , fermentation , hydrolysis , biofuel , steam explosion , food science , microbiology and biotechnology , biochemistry , engineering , biology
The main by‐product of the alcohol industry is the sugarcane bagasse, which can be used to produce second generation ethanol. Studies about pretreatment technologies are increasingly greater, a fundamental step for the efficient this fuel production. The reagents and wastewater treatment costs are still a bottleneck for this type of technology. The present work aimed to study the pretreatments of sugarcane bagasse using a highly oxidative solution Ox‐B. In this study we used high solids loadings, aimed at reducing the use of reagent and the water consumption. We carried out an experimental design 3 2 for the pretreatment step. The pretreated material followed an enzymatic hydrolysis with 10% (m/v) loading, and enzymatic loading of 15 Filter Paper Units (FPU)/g and 25 cellobiose units (CBU)/g of bagasse. For the best pretreatment condition the desirability tool showed the best pretreatment condition for the release of glucose as 20% solids, 25°C and 2 h, achieving 251.94 mg of glucose per gram of raw bagasse. However, the maximum ethanol yield was 70%, that possible was affected by the presence of chlorine in the pre‐treated mass that has been affected the fermentation process. In general, Ox‐B proved to be a potential pretreatment agent for sugarcane bagasse. Practical Applications Sugarcane bagasse is a residual material which can be used for second‐generation (2G) bioethanol production. In this study, Ox–B pretreatment of sugarcane bagasse using high solid loadings, both in the pretreatment (20–40% w/v) and the enzymatic hydrolysis (10–20% w/v) stages was conducted in order to reduce reagent usage and consumption of water. The studies will be useful for ethanol production from lignocellulose.