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Establishment of an Enzymatic Membrane Reactor for Angiotensin‐Converting Enzyme Inhibitory Peptides Preparation from Wheat Germ Protein Isolates
Author(s) -
He Ronghai,
Xing Huan,
Wang Zhiping,
Ding Wenhui,
Zhu Peipei,
Liu Bin,
Ma Haile
Publication year - 2016
Publication title -
journal of food process engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 45
eISSN - 1745-4530
pISSN - 0145-8876
DOI - 10.1111/jfpe.12224
Subject(s) - chemistry , substrate (aquarium) , enzyme , hydrolysis , chromatography , membrane , membrane reactor , angiotensin converting enzyme , hydrolysate , enzymatic hydrolysis , biochemistry , product inhibition , permeation , protease , non competitive inhibition , biology , ecology , endocrinology , blood pressure
In this paper, an efficient enzymatic membrane reactor ( EMR ) was established for the preparation of angiotensin‐converting enzyme ( ACE ) inhibitory peptides from wheat germ protein isolates ( WGPI ). It promoted the protein conversion and improved the enzyme utilization rate. ACE inhibitory peptides of WGPI were prepared using 5 kDa membrane device for enzyme membrane coupling. Based on the results of the protease activity preservation experiment, substrate intercept experiment, batch EMR experiment and continuous EMR experiment, the conditions of EMR were decided as follows: substrate concentration of 1.2% (w/v), alcalase quantity of 110 μ L , temperature of 50 C , pH of 9.0, effective volume of 0.4 L , permeation flux of 0.005 L /min and turn into 0.011 L /min after 10 min and runtime of 300 min. The continuous EMR method showed high conversion rate of protein (55.3%) and IC 50 of peptides (0.50 mg/m L ). The conversion rate of protein significantly increased by 36.17% and the IC 50 of peptides significantly reduced by 30.6% compared with the traditional enzymatic hydrolysis method. Therefore, the continuous EMR is beneficial to the efficient production of ACE inhibitory peptides from WGPI . Practical Applications Using food protein as raw material, functional polypeptides products have been developed by employing enzymatic hydrolysis and series of high technologies. However, during the preparation of functional polypeptides, some problems of low reaction efficiency, product yield and product activity existed, which seriously influence the performance of technology industrialization. The enzymatic membrane reactor ( EMR ) is an effective mode which can achieve continuous operation by the integration of substrate enzymolysis, enzyme recovery and hydrolyzate separation in functional polypeptides producing. Utilization of the EMR technology will not only increase food protein commercial value, but will also satisfy the needs of the polypeptide processing industries for efficient, low‐cost producing.