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Extraction Techniques and Stability of Carotenoprotein from Carrot ( D aucus carota L .) Root
Author(s) -
Zhao Qiaojiao,
Zhang Weiwei,
Wu Yanwen,
Ouyang Jie
Publication year - 2015
Publication title -
journal of food process engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 45
eISSN - 1745-4530
pISSN - 0145-8876
DOI - 10.1111/jfpe.12134
Subject(s) - chemistry , daucus carota , carotenoid , extraction (chemistry) , hydrolysis , chromatography , pigment , food science , biochemistry , botany , biology , organic chemistry
Abstract The key technological parameters for the extraction of water‐soluble carotenoprotein from carrot root by enzymatic hydrolysis and partial characteristics of carotenoprotein were studied. The extraction yield of carotenoprotein reached 11.99 μ g β‐carotene equivalent/g fresh weight by adding 50 g of chopped carrot into 100 mL of S pan 40 solution (0.1%, w/v) followed by homogenizing, then 1.5 g of cellulase and 0.4 g of pectinase were added to hydrolyze at 40C for 120 min, and the mixture was finally treated with sonication for 40 min. Purification was carried out by ammonium sulfate precipitation, followed by S ephadex G ‐200 gel chromatography. The molecular weight ( MW ) of the carotenoprotein was 419 kD and the MW s of subunits were 39, 22 and 15 kD. Stability comparison between water‐soluble carotenoprotein and oil‐soluble carotenoid indicated that the stabilities of carotenoprotein were better than those of carotenoid ( P < 0.001), when exposed to light or heat treatments. Practical Applications As a kind of natural pigment in plants, carotenoid has many functions, such as antitumor effect, precursor of vitamin A, oxidation resistance and the ability to enhance immune response. Commercial interest of producing carotenoid is increasing too. However, the shelf life of carotenoid achieved by traditional solvent extraction is affected because of bad stability. This paper aimed to extract water‐soluble carotenoprotein from carrot by enzymatic hydrolysis, research the biochemical characteristics of carotenoprotein and compare the stability between carotenoid and carotenoprotein. Optimization of the extraction techniques could be considered as a sustainable alternative method to achieve pigment and improve its stability. We believe that pigment industry can utilize the results of this study in their applications.