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Ozone nanobubble treatments improve survivability of Nile tilapia ( Oreochromis niloticus ) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila
Author(s) -
Thanh Dien Le,
Linh Nguyen Vu,
Sangpo Pattiya,
Senapin Saengchan,
StHilaire Sophie,
Rodkhum Channarong,
Dong Ha Thanh
Publication year - 2021
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13451
Subject(s) - nile tilapia , oreochromis , aeromonas hydrophila , tilapia , biology , gill , aquaculture , microbiology and biotechnology , bacteria , fish mortality , immunity , veterinary medicine , immune system , fish <actinopterygii> , fishery , immunology , medicine , genetics
A rapid increase in multi‐drug‐resistant (MDR) bacteria in aquaculture highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB‐O 3 ) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple treatments with direct NB‐O 3 exposures caused alterations to the gills of exposed fish. Here, we set up a modified recirculation system (MRS) assembled with an NB‐O 3 device (MRS‐NB‐O 3 ) to investigate whether MRS‐NB‐O 3 (a) were safe for tilapia ( Oreochromis niloticus ), (b) were effective at reducing bacterial load in rearing water and (c) improved survivability of Nile tilapia following an immersion challenge with a lethal dose of MDR Aeromonas hydrophila . The results showed no behavioural abnormalities or mortality of Nile tilapia during the 14‐day study using the MRS‐NB‐O 3 system. In the immersion challenge, although high bacterial concentration (~2 × 10 7 CFU/ml) was used, multiple NB‐O 3 treatments in the first two days reduced the bacteria between 15.9% and 35.6% of bacterial load in water, while bacterial concentration increased from 13.1% to 27.9% in the untreated control. There was slight up‐regulation of non‐specific immune‐related genes in the gills of the fish receiving NB‐O 3 treatments. Most importantly, this treatment significantly improved survivability of Nile tilapia with relative percentage survival (RPS) of 64.7% – 66.7% in treated fish and surviving fish developed specific antibody against MDR A. hydrophila . In summary, the result suggests that NB‐O 3 is a promising non‐antibiotic approach to control bacterial diseases, including MDR bacteria, and has high potential for application in recirculation aquaculture system (RAS).