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The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida
Author(s) -
Ye Tingqi,
Mu Cuimin,
Chen Jiakang,
Pan Guangchen,
Wang Xuepeng
Publication year - 2021
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13298
Subject(s) - virulence , biology , gene expression , gene , microbiology and biotechnology , mutant , regulation of gene expression , genetics
Edwardsiella piscicida ( E. piscicida ) is an important fish pathogen. However, the mechanism of Glu6P transport regulatory protein UhpA how to affect the virulence gene expression in E. piscicida is still unclear. The results in this study showed that the metabolism‐related gene expression of cysteine synthase (orf 1134) and sulphate transporter ( ych M) in the uhp A mutant strain Δ uhpA was 0.76‐fold and 0.68‐fold lower than the ones in the wild strains ( p < .05). The gene expression of ethA and ethB in the Δ uhp A strain was 0.80‐fold and 0.72‐fold lower than the ones in the wild strains ( p < .05). However, the gene expression of fliC and flgN in the Δ uhpA was 1.51‐fold and 1.21‐fold higher than the ones in the wild strains ( p < .05). The gene expression of T3SS ( esrB and esrC ) and T6SS ( evpB and evpC ) in the Δ uhpA was 1.27‐fold, 1.13‐fold, 1.28‐fold and 1.23‐fold higher than the ones in the wild strains ( p < .05). This suggested that the uhp A gene could regulate the key virulence gene expression, and the uhp A gene was associated with the pathogenicity of E. piscicida in fish.