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Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells
Author(s) -
Ford Lorelei,
Subramaniam Kuttichantran,
Waltzek Thomas B.,
Bowser Paul R.,
Hanson Larry
Publication year - 2021
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13278
Subject(s) - ictalurus , catfish , biology , ictaluridae , cytochrome c oxidase , cell culture , ovary , gene , microbiology and biotechnology , fishery , zoology , fish <actinopterygii> , genetics , mitochondrion
Abstract The channel catfish ( Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the standard cell line used for channel catfish diagnostics. Next‐gen sequencing studies of a virus cultured in the CCO cells revealed mitochondrial sequences matching those of brown bullhead ( Ameiurus nebulosus, Lesueur). Therefore, we systematically performed partial cytochrome oxidase 1 gene sequencing of several sources of the CCO cell line and all matched the brown bullhead and not the channel catfish.