Premium
Characterization and functional analysis of a galectin‐related protein B from Nile tilapia involved in the immune response to bacterial infection
Author(s) -
Niu Jinzhong,
Luo Guoling,
Liu Xinchao,
Huang Yu,
Tang Jufen,
Wang Bei,
Lu Yishan,
Cai Jia,
Jian Jichang
Publication year - 2021
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13273
Subject(s) - nile tilapia , biology , streptococcus agalactiae , galectin , immune system , oreochromis , open reading frame , agglutination (biology) , microbiology and biotechnology , edwardsiella tarda , aeromonas hydrophila , spleen , peptide sequence , bacteria , antibody , immunology , biochemistry , streptococcus , gene , fish <actinopterygii> , genetics , fishery
Galectin‐related protein is a kind of lectin without canonical activity that regulates cell adhesion and cell growth. In this study, a novel galectin‐related protein B ( OnGRPB ) was identified from Nile tilapia ( Oreochromis niloticus ). The open reading frame of OnGRPB was 438 bp and encoded a peptide of 145 amino acids. The deduced protein sequence of OnGRPB possessed a conserved carbohydrate recognition domain (CRD) with partial sugar binding sites (N‐R, V‐N and W‐E) and shared high identities with other fish GRPB proteins. The qRT‐PCR analysis found that OnGRPB was widely distributed in various tissues and monocyte/macrophages (Mo/MΦ) of healthy tilapia. After Streptococcus agalactiae infection, OnGRPB transcripts were significantly up‐regulated in liver, spleen, head kidney and Mo/MΦ. The recombinant OnGRPB protein (rOnGRPB) had the binding activity and agglutination ability to bacteria. Also, rOnGRPB could modulate antibacterial activity and inflammatory factor expression of Mo/MΦ. These data collectively indicate that OnGRPB plays roles in the immune response of Nile tilapia against bacterial infection.